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Fig. 1 | BMC Cancer

Fig. 1

From: RETRACTED ARTICLE: KRAS activation in gastric cancer stem-like cells promotes tumor angiogenesis and metastasis

Fig. 1

Conditioned media from GA spheroid cells promotes angiogenesis. A Tube formation assay using Mouse Primary Vein Endothelial Cells (MVECs) in conditioned medium from Tcon3944 mouse GA cells grown as monolayers and spheroids with basal media devoid. B Tube formation assay using Human Umbilical Vein Endothelial Cells (HUVECs) in conditioned medium from human GA cells grown as monolayers and spheroids. C Western blot for KRAS in spheroid cells transduced with KRAS shRNA (sh.KRAS) and control scramble shRNA (sh.Scr). β-actin was used as a loading control (Original version in Suppl. Figs. S5A, B). D-E Tube formation assay of MVECs or HUVECs in conditioned media from spheroids transduced with sh.KRAS and sh.Scr. Scale bar, 50m. Bars represent standard deviation. *p < 0.05 compared to control

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BMC Cancer

ISSN: 1471-2407

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