Fig. 3
An acid phosphatase assay (A) was used to measure the viability of the Hs578Ts(i)8 cells after 48 h of inhibitor treatment. Representative dot plots are shown for annexin/PI flow cytometry (B) which was used to confirm toxicity at the chosen drug concentrations. Staurosporine (1 µM) was used as a positive inducer of apoptosis. Table (B) shows results from annexin V/PI flow cytometry. (D) A quick screening assay was developed in which cells were cultured in a 24-well plate and treated with drugs/compounds for 48 h. Afterwards, CM was collected and analysed for the presence of CD9 using imaging flow cytometry. CD9 + particles were detected and normalised to 1 × 104 (D) Hs578Ts(i)8 cells. Tables show mean ± SEM of n = 3 independent biological experiments. Graphs represent mean ± SEM of n = 4 independent biological experiments. One-way ANOVA was used as statistical test. ***P < 0.001, ****P < 0.0001