Fig. 2
EV sub-populations (A) yield, (B) size were measured by NTA and (C) protein content by Bradford assay. Immunoblotting (D) was performed, with Hs578Ts(i)8 cell lysate used as control. WCL = Whole cell lysate. TEM analysis (E) was also performed. Hs578T cells were treated with Hs578Ts(i)8-derived heterogeneous EVs and EVs’ sub-populations and a migration assay was performed. Scale bars on TEM images are 500 nm (low magnification) and 100 nm (high magnification). Full-length blots/gels are presented in Supplementary Fig. 2. Graph (F) represents mean ± SEM of n = 4 independent biological experiments. One-way ANOVA was used as a statistical test for all graphs. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001