Fig. 5
MMP7 and MMP12 expression in UBC patient biopsy tissues and an autochthonous murine urothelial cancer model. Normalised gene expression levels in tumour samples (n = 306) were analysed with microarray probes for MMP7 (A) and MMP12 (B) in relation to the patient’s stage. Kaplan–Meier plots for selected patients (n = 224) of the aforementioned cohort based on high or low mRNA levels of MMP7 (C) and MMP12 (D). Mmp12 mRNA expression at the single-cell level from a murine urothelial cancer model is significantly higher at the MIBC stage, irrespective of sex (E). Bar plots illustrate the percentage and the total number of macrophages exhibiting non-zero expression of Mmp12 (F) for the different stages and sexes. The average log2 fold changes and the BH-adjusted p-values were computed using limma for the microarray data, while the respective fold changes and Bonferroni-corrected p-values were calculated through a Wilcoxon rank-sum test provided by Seurat for the single-cell RNA-sequencing data