Fig. 4

Variant effector predictions of mitochondrial DNA in the colorectal cancer patients. Distribution of mutation types in coding sequences of whole blood (WB), peripheral blood mononuclear cells (PBMC), plasma extracellular vesicles (EV), and formalin-fixed paraffin-embedded (FFPE) or fresh-frozen (FF) tumor specimens in (A) rectal (n = 8) and (B) colon (n = 8) cancer patients. Missense mutation burden (in %) of WB, PBMC, plasma EV, and FFPE tumor specimens from (C) rectal cancer (n = 8) patients and WB, plasma EV, and FF tumor specimens from (D) colon cancer (n = 8) patients. Points connected with a line represent matched patient specimens. The mean number of all variants detected in each sample type was compared to the EV number by Dunnett´s multiple comparison (*, p = 0.033; **, p = 0.0021; ***, p = 0.0002; ****, p < 0.0001)