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Table 1 Primer sequences for PCR amplification of the target regions in G6PD gene

From: Hemoglobinopathies, merozoite surface protein-2 gene polymorphisms, and acquisition of Epstein Barr virus among infants in Western Kenya

 

Mutations

Substitutions

Primer sequence (5’- 3’)

Size (bp)

Group A1

Viangchan

Chinese

871 G > A

1024 C > T

CCAACTCAACACCCAAGGAGC

GGCATGCCCAGTTCTGCCTTG

280

Group A2

Mahidol

Coimbra

487 G > A

592 C > T

TGAATGATGCAGCTCTGATCC

CCAGGTGAGGCTCCTGAGTAC

293

Group B

Union

Kaiping

1360 C > T

1388 G > A

TGGCATCAGCAAGACACTCTC

GGAGAGGCATGAGGTAGCTCC

384

  1. G6PD mutation types and G6PD primer sequences. Target region were amplified based on basepair (bp) sizes