Fig. 2
From: AQP8 promotes glioma proliferation and growth, possibly through the ROS/PTEN/AKT signaling pathway
Cell clone and EDU experiments showing the proliferation capability of A172 and U251 cells infected by different viruses. (A, B) Cell clone experiment. The colony formation capability of A172 and U251 cells is increased by AQP8 overexpression (O.E) and decreased by AQP8 knockdown (K.D) compared with the control group. (C, D) Bar graphs of colony formation of A172 and U251 cells. Complete proliferation analysis was performed by observing the number of cell clones (vs. control, *p < 0.05, **p < 0.01). (E, F) EDU fluorescence staining experiment. Red represents EDU, and blue represents nucleus. Fluorescence intensity in A172 and U251 cells is increased by AQP8 overexpression and decreased by AQP8 knockdown. (G, H) EDU percentage and analysis in A172 and U251 cells of different groups (vs. control, *p < 0.05, **p < 0.01). EDU, 5-ethyl-2-deoxyuridine; AQP, aquaporin; NC, negative control; O.E, AQP8-overexpressed; K.D, AQP8-knockdown; DAPI, 4,6-diamino-2-phenylindole. All experiments were performed in triplicate