Fig. 1

Screening and identification of viruses with overexpressed and knocked down AQP8. A-B Confirmation of the expression of AQP8 in A172 and U251: (A) The expression level of AQP8 in A172 and U251 cells. (B) The comparison of AQP8 protein content (ns, no significant difference). C-D Virus screening: (C, D) relative mRNA expression analysis of AQP8 in all groups, including one negative control, three overexpression and three knockdown groups of AQP8 (vs. control, *p < 0.05, **p < 0.01). Viruses d and f have better effects on overexpression and knockdown in both cell types. E-H NC virus screening for an overexpressed virus and a knockdown virus by western blot: (E) Expression of AQP8 in different groups after A172 cell infection. (G) Bar graph of the relative expression of AQP8 in A172 cells. (E) Expression of AQP8 in different groups after U251 cell infection. (H) Bar graph of the relative expression of AQP8 in U251 cells. AQP, aquaporin; NC, negative control; O.E, overexpressed; K.D, knockdown. All experiments were performed in triplicate