Fig. 5
From: The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study
Breast carcinogenesis in transgenic mice with tissue-specific abrogation of PTEN. Primers were designed targeting the PTEN gene to identify the deletion of exon 5 (A). Different tissues and tail samples from PGC-cre/PTENf/f mice were subjected to DNA extraction and PCR amplification using the above-mentioned primers (B). PTEN deletion was also seen in the lung, stomach, kidney, and breast using PCR(C) and immunohistochemistry (IHC, D). Histologically, we observed gastric cancer and identified the breast tumor as lobular carcinoma and immunohistochemically as triple-negative breast cancer (E). Note: WT, wild-type; P, primer; F, forward; R, reverse; NC, negative control; HE, hematoxylin–eosin