Fig. 4

Expression and activity of cre in PGC-cre transgenic mice. Transgenic cre mice were established according to the schematic diagram using the PGC promoter (A). We identified nine cre-positive founders using PCR of mouse tail DNA (B). Cre expression levels were detected by real-time RT-PCR (C), western blot (D), and immunohistochemistry (E). PGC-cre/B6/JGpt-H11em1Cin (CAG-LoxP-ZsGreen-stop-Loxp- tdTomato)/Gpt (abbreviated as B6-G/R) mice were genetically screened by PCR of mouse tail DNA (F). The red fluorescence to indicate cre activity was seen in gastric, bronchoalveolar, kidney tubular, ductal and lobular epithelial cells of PGC-cre/B6-G/R mice, but green fluorescence was seen in the B6-G/R mice (G). Note: PC, positive control; NC, negative control; WT, wild-type C57 mouse