Fig. 2

Effects of PGC KO on the phenotypes of transgene mice. PGC knockout (PKO) mice were generated according to the schematic diagram using the CRISPR/Cas9 technique (A). Wild-type (WT) and homozygous (H) PKO mice were genetically identified by PCR of mouse tail DNA (B). PGC expression loss was seen in PKO mice compared with WT mice according to RT-PCR, western blot, and immunohistochemistry (C). Body weight and length were compared between WT and PKO mice (D). Survival curves of these three types of mice were plotted using the Kaplan-Meier method (E). Routine blood test (F) and hepatic and renal function tests (G) were compared between the WT and PKO mice. Note: E, exon; M, marker; NC, negative control; PC, positive control; RT-PCR, reverse transcription- polymerase chain reaction; WB, western blot; WBC, white blood cell; RBC, red blood cell; Plt, platelet; Mon, monocyte; Hb, hemoglobin; N/Gr, neutrophil/granulocyte; ALP, alkaline phosphatase; UA, uric acid; Alb, albumin