Fig. 1

Expression of ANGPTL4 is associated with patient outcomes and modifies expression of resistance-associated genes. (a) A Kaplan Meier curve for overall survival using TCGA-PAAD data. The patients with the top (red) and bottom (blue) 25% average ANGPTL4 gene expression have significantly different OS. (p = 0.043, log-rank test) (b) Relative expression of ANGPTL4 transcript in MP2_ANGPTL4_KD (black circles), MP2 (pink squares), and MP2_ANGPTL4_OE (turquoise triangles) as measured by qPCR. P-values from unpaired, two-tailed parametric t-tests performed with 95% confidence intervals are displayed. Values are normalized with ACTB as the housekeeper. (P-values ≤ 0.0001=****) (c) Heatmap displaying RNA-seq results from MP2_ANGPTL4_KD (black), MP2_NTC (pink), and MP2_ANGPTL4_OE (turquoise) cell lines. Displayed are 15 genes associated with both ANGPTL4 overexpression and gemcitabine resistance. Normalized expression for each of the 15 genes is plotted in the heatmap. Red (*) indicates ITGB4 used for later experiments