Fig. 7

Colocalization and clinical parameter correlation of NPY1R, NPY5R, and CAIX in human breast carcinomas. Immunofluorescence of NPY1R, NPY5R, and CAIX (hypoxia marker) was performed on 46 human breast tumor and 10 normal breast tissue sections. A minimum of 4 immunofluorescence images per slide (section) were evaluated using Fiji for ImageJ with the JACOP plugin. Proteins levels were quantified and Manders overlap coefficient was calculated for NPY1R or NPY5R with CAIX. Data was then presented as violin plots based on the patient parameters of (A-E) Pathological T, (F-J) Clinical M, (K-O) and Grade. (D) Samples were classified by current patient status (alive or deceased) and correlated to the protein abundance of NPY1R (P), NPY5R (Q) and CAIX (R). Kaplan-Meier plots assessing progression-free survival of breast cancer patients grouped into high and low NPY1R (S) or NPY5R (T) expression using the median expression between the two groups as a threshold. Error bars represent the SEM. and * represent p < 0.05 using a one-way ANOVA and Tukey’s HSD post-hoc test