Fig. 6

NPY5R protein levels, but not NPY1R, are higher and colocalize with hypoxia more in metastatic tumors relative to non-metastatic cancer and normal tissue. Representative immunofluorescence images of human normal (n = 10) and cancer (n = 46) breast tissue sections used in the analyses for Fig. 7. The 46 breast tumor samples were divided into M0 (non-metastatic; n = 35) and M1 (metastatic; n = 11). These breast tumor samples had associated stage, grade, receptor status, and patient outcome information and were also used in the analyses in Fig. 7 and Figure S1. CAIX was used as a marker of hypoxia. DAPI was used as a marker of cells (nuclei). Scale bar, 100 μm