Fig. 4

Antagonizing NPY5R reduces MCF7 spheroid growth and invasion. Growth of spheroids was measured in (A-C) MDA-MB-231 and (D-F) MCF7 cells treated with subtype-specific NPYR antagonists (Y1^- or Y5^-) following stimulation with agonist. Data (n ≥ 4) represent the mean growth of spheroids over 24 h relative to vehicle control. Invasive protrusions of spheroids encased in Matrigel were measured in (G-I) MDA-MB-231 and (J-L) MCF7 cells treated with subtype-specific NPYR antagonists (Y1^- or Y5^-) following agonist stimulation over a time course of 96 h. A general agonist that stimulates all NPYR subtypes (NPY) or subtype-specific agonists (Y1⁺ or Y5⁺) were used. All conditions are normalized to their respective vehicle control. The Bartlett test was used to verify that all data sets were normally distributed. Box plots (A-F) and line graphs (G-L) have error bars that represent the SEM and * represent p < 0.05 when an antagonist condition was significantly different compared to agonist alone using a one-way ANOVA and Tukey’s HSD post-hoc test