Fig. 6
From: High HSPB1 expression predicts poor clinical outcomes and correlates with breast cancer metastasis
Transient knockdown of HSPB1 affects breast cancer cell migration, invasion, and apoptosis. A The effect of transient knockdown of HSPB1 expression on the cell migration was determined by using the wound healing assay. The quantification of each analysis is shown in the right figure. The experiments were carried out in triplicate (**p < 0.01). The scratch area was calculated using Image J software. Cell scratch area (0 h) minus cell scratch area (72 h) to get the cell migration area, the percentage of cell migration area to cell scratch area (0 h) is the cell migration index. B Transwell® assay was used to determine the cell invasion after HSPB1 transient knockdown. The quantification of each analysis is shown in the right figure. The experiments were carried out in triplicate (***p < 0.001). C The impact of transient knockdown HSPB1 expression on cellular apoptosis as determined via flow cytometry. All assays were performed in triplicate. SK-BR-3 and MDA-MB-231 breast cancer cell lines were used in this study. The right picture shows the percentage of cell apoptosis. The experiments were carried out in triplicate (***p < 0.001)