Fig. 6

Gene-length associated bias in snRNA-seq. A Comparison of dissociation protocol impact on percentage of reads mapping to exonic, intronic, and intragenic regions with B summary plot averaging all sarcoma subtypes and comparing between protocols. C snRNA-seq samples were enriched in genes with longer gene length. Genes were split into quartiles based on gene length. D Increased PolyA regions were associated with longer gene lengths. E Boxplots display the data distributions (centerline: median, box limits: first and third quartiles, and the whiskers are the highest and lowest values that are no greater and smaller than 1.5 × the interquartile range) of the Warm Dissociation scores, which was significantly higher in the Warm and Cold protocol as opposed to the Nuclei protocol. Length Bias score was significantly higher in the Nuclei protocol (Wilcoxon test, **** denotes p <  = 0.0001)