Fig. 3From: Dissociation protocols used for sarcoma tissues bias the transcriptome observed in single-cell and single-nucleus RNA sequencingPerformance of each protocol on various sarcoma subtypes. A UMAP embedding of all cells labeled by Sarcoma subtype, Tissue state, and Dissociation type. B Boxplots display the data distributions (centerline: median, box limits: first and third quartiles, and the whiskers are the highest and lowest values that are no greater and smaller than 1.5 × the interquartile range) for the enrichment of selected stress-response genes in the Warm dissociation protocol and selected lncRNA in the Nuclei isolation protocol. Post hoc comparison with Bonferroni correction found statistically significant differences for all comparisons for both stress-response and lncRNA genesBack to article page