Fig. 2

Patient information and quality control metrics. A Overview of all sarcoma subtypes that were processed and evaluated. For each sample, the number of cells or nuclei passing QC thresholds, the number of sequencing reads per cell/nuclei, the number of genes per cell/nuclei, and the median percentage of UMIs mapping to mitochondrial genes are displayed in the table. All samples had less than 0.01 doublet fraction. B Boxplots display the data distributions (centerline: median, box limits: first and third quartiles, and the whiskers are the highest and lowest values that are no greater and smaller than 1.5 × the interquartile range) for number of UMIs per cells/nuclei, number of genes per cell/nuclei, and percentage of UMIs mapping to mitochondrial genes vary across sarcoma subtypes and choice of protocol. The dots represent the mean value for each PDX, where the color denotes the dissociation protocol as displayed in the legend