Fig. 6

Platelet production induced by 2R13 in mice. a Schematic of the experimental design. BALB/c mice were subcutaneously injected with 0.1% BSA-PBS (control) or rhTPO (50 ng) for seven consecutive days, or once with 2R13 (0.1, 1, 2, or 10 μg). Blood was collected on the indicated days over 14 days. To analyze BM-derived LSK⁺ cells, BALB/c mice were subcutaneously injected with 0.1% BSA-PBS (control) or rhTPO (50 ng) for seven consecutive days, or once with 2R13 (10 μg). On day 7 post-initiation of treatment, BM cells were obtained after sacrificing the mice and were incubated with LSK⁺ cell markers (PerCP-Cy5.5-labeled lineage antibody cocktail, anti-Sca-1-FITC antibody, and anti-c-kit-APC antibody), following which they were analyzed using flow cytometry. Quantification of b platelet and c WBC counts. Data are the absolute count mean ± SD (n = 4/group). d Flow cytometric analysis of BM-derived LSK⁺ cells. Data are the mean ± SD (n = 4/group). The unpaired t-test was used for statistical analysis. **p < 0.01 and ***p < 0.001 vs control; #p < 0.05, ##p < 0.01, and ###p < 0.001 vs rhTPO