Fig. 5
From: Thrombopoietin receptor agonist antibody for treating chemotherapy-induced thrombocytopenia
MPL signal transduction by 2R13 in human platelets. Western blotting of human platelets stimulated with rhTPO or 2R13. a rhTPO or 2R13 was added at the indicated concentrations for 30 min. b rhTPO (50 ng/mL) or 2R13 (300 ng/mL) was added over the indicated period. Total lysates were analyzed by immunoblotting with the indicated antibodies and β-actin was used as a loading control