Fig. 4

Effect of 2R13 on MK polyploidization in PB-CD34⁺ cells. PB-CD34⁺ cells were stimulated with rhTPO or 2R13 at the indicated concentrations for 13 days. Differentiated cells were analyzed using flow cytometry with anti-CD41a-FITC-conjugated antibody, followed by propidium iodide staining. a Ploidy status of CD41a⁺ cells. b Representative flow cytometric profile of ploidy levels. c, d Percentage and number of high-ploidy (≥ 8N) MKs. The number of cells produced per input of PB-CD34⁺ cells was calculated by multiplying the number of total nucleated cells with the percentage of high-ploidy CD41a⁺ cells. Data are the mean ± SD (n = 3). One-way and two-way ANOVA were used for statistical analysis. *p < 0.05, **p < 0.01, and ***p < 0.001 vs control; #p < 0.05, ##p < 0.01, and ###p < 0.001 vs rhTPO