Matrigel-based organoid culture of malignant mesothelioma reproduces cisplatin sensitivity through CTR1

BMC Cancer

Table 1 Primer sequences for cloning and mutagenesis of mCTR1

Primer	Primer orientation	Nucleotide sequence
N-terminal Flag mCTR1	Forward	5'-GAATTCGCCACCATGGACTACAAAGACGATGACGACAAGAACCA-3'
N-terminal Flag mCTR1	Reverse	5'-GGATCCTCAATGGCAGTGCTCTGTGA-3'
mCTR1	Forward	5'-GAATTCGCCACCATGAACCATATGGGGATGAACCATATGG-3'
mCTR1	Reverse	5'-GGATCCTCAATGGCAGTGCTCTGTGA-3'
N23D	Forward	5'-ATTACCATGCCACCTCACCACCAC-3'
N23D	Reverse	5'-GTCGTCGTCCGTGTGGTTCATACCCATATG-3'
N23Q	Forward	5'-ATTACCATGCCACCTCACCACCAC-3'
N23Q	Reverse	5'-CTGGTCGTCCGTGTGGTTCATACCCATATG-3'

Primers were designed for generating different mouse CTR1 constructs, which were ligated into the appropriate epitope-tag sequences and then cloned into pcDNA3.2 vector

ISSN: 1471-2407