Fig. 5

Matrigel mediates high sensitivity to cisplatin. a Phase-contrast images of m5 MM-organoids before and after cisplatin (100 μg /ml, 24 h) treatment. The majority of MM organoids die within 24 h after cisplatin treatment. b, c Cell viability of m5/m107 MM-organoids and 2D MM cell lines in different cisplatin dosage (0.5–200 μg/ml, 24 h). IC50 values (concentration responsible for 50% cell growth inhibition) are shown in each line. Green lines, MM-organoid; black lines, 2D MM-cell lines. d Slc31a1 (Ctr1) expression level in MM-organoids and 2D MM cell lines from RNA-sequence data. e H&E staining (upper) and immunofluorescent images from m5 MM primary tissue sample, SC injection and organoids. CTR1 (Alexa488, green); nuclear staining (Hoechst33342, blue; scale bar = 20 μm). f Immunoblot analysis of m5/m107 MM-organoids (3D) and 2D MM cell lines (2D); g, glycosylation (30–50 kDa); CTR1 (25 kDa); t, truncate (15 kDa). g, h Enrichment analysis using DAVID indicates significant activation of protein glycosylation and lipidation pathways between mesothelioma 2D- and 3D-cultures