Fig. 3
Subcutaneous (sc) injection of MM-organoids and 2D MM cell lines. a SC inoculation to nude mice with 2D MM cell lines (m107), left (L), and MM-organoids, right (R), respectively. Mice were euthanized at day 36 after inoculation. Left image, whole-body image from the back. The right upper image, lateral view of the tumor. The right lower image, intradermal view of the tumor. Arrowheads and red lines, tumor (scale bar = 10 mm). b Histological images of the H&E-stained right tumor nodule by m107 cell line (scale bar 20 μm). I, invasion; T, tumor nodule. c Left (2D MM cell lines) and Right (MM-organoid) tumor size (mm.2). d Histological presentations with immunohistochemical staining of mesothelial markers (WT-1, MSLN). Left images, m5 mice origin. Right images, m107 mice origin (scale bar 100 μm)