Fig. 1

Preparation of malignant mesothelioma (MM) organoids. a–c Kaplan Meier analysis for the MM mouse models, describing the overall survival probability in p53( +/-); blue line, crocidolite group; red line, NT-50 group; gray blue lines, the untreated control groups. d, e Time course culture of the epithelioid (m12) and sarcomatoid (m5) MM organoids in 1–7 days (scale bar = 50 μm). f Schematic illustration of the protocol to establish MM organoids from the dissection of mouse MM to matrigel-based 3D culture. g Phase-contrast and H&E-stained images of normal mesothelial cell or MM-2D and 3D cultures (scale bar = 100 μm)