Fig. 6

Treg and Tc in tumors formed by SCCVII/SF-GFP (SCC), D2, and D3 cells. (A). FoxP3 IHC was used to show Treg in the tumor tissue sections. (B). CD8 IHC was used to show Tc cells in the tumor tissue sections. (C) Upper panel: flow cytometry shows Treg (CD4 + CD25 + FoxP3+) in the upper right quadrant. The top label shows GFP + percentages in the tumor. Lower panel: Quantified results of Treg/GFP + ratio, Treg percentage in total cells, and Treg percentage in helper T cells. (D) Upper panel: flow cytometry shows Tc (CD45 + CD8+) in the upper right quadrant. The top label shows GFP + percentages in the tumor. Lower panel: Quantified results of Tc/GFP + ratio, Tc/total cell ratio, and Tc percentage in total leukocytes (CD45+). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Representative data of biological replicates are shown for flow analysis