Fig. 5

TAM in tumors formed by SCCVII/SF-GFP (SCC), D2, and D3 cells. (A) F4/80 IHC was used to stain TAM in the tumor tissue sections. (B) Flow cytometry analysis of TAM. Left panel: TAM (CD45 + CD11b + F4/80+) are shown in the upper right quadrant of the flow chart. Top label shows GFP + percentages in the tumor. Right panel: Quantified results of TAM/GFP + ratio (top) and TAM percentage in total leukocytes (CD45+) (lower). (C) Flow cytometry analysis of TAM polarization. Left panel: M2 TAM (CD11b + F4/80 + Arg1+) are shown in the upper right quadrant of the flow chart. Right panel: The percentages of M1 and M2 cells in total TAM (top). The percentages of M1 and M2 cells in total cells (sum of tumor and non-tumor cells). Representative data of biological replicates were shown for flow analysis. **p ≤ 0.01, ***p ≤ 0.001