Fig. 1

In vivo and in vitro fusion between myeloid and SCCVII/SF cells. Representative data are shown. (A) Flow cytometry analysis of tumors derived from SCCVII/SF-H2bGFP grown in C3H mice. The tumor cell suspensions were stained with appropriate antibodies (indicated in the text). Left panel: The tumor cells, gated by high GFP expression, were analyzed for co-expression of myeloid cell markers CD45 and CD11b. The proportion of CD45⁺CD11b⁺ expression in the tumor cells is indicated in the upper right quadrant. Right panel: Images of in vivo fusion between SCCVII/SF-H2bGFP cells and myeloid cells were captured by imaging flow cytometry. The tumor cell suspensions were stained with CD11b and DAPI. The double-positive hybrid cells exhibited nuclear GFP and membranous CD11b. (B) Co-culture of SCCVII/SF-H2bGFP cells and BMM formed multinucleated giant cells containing GFP + PU.1 + nuclei. Arrows point to double-positive nuclei