Fig. 7

Knockdown of TIMM50 suppressed EC cell proliferation, migration, and lactate synthesis. a, b qRT-PCR and Western blot analysis of TIMM50 expression in EC cells(The samples derive from the same experiment and that gels/blots were processed in parallel). c, d qRT-PCR and Western blot analysis of TIMM50 expression in Ishikawa cells and HEC-1B cells after transfection of the shRNA vector(The samples derive from the same experiment and that gels/blots were processed in parallel). e, f, g CCK-8, EdU binding and colony formation assays were performed to evaluate the proliferation of Ishikawa cells and HEC-1B cells. h Transwell assays were conducted to assess the migration of Ishikawa cells and HEB-1C cells. i Lactate and glucose assays were used to evaluate the lactate metabolism of Ishikawa cells and HEB-1C cells. Scale bar, 100 μm. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001