Fig. 3
Cytotoxic activity of GemHCl and 4NSG-SLN against PPCL-192, PPCL-135, PPCL-46, and PPCL-68 cells at varying concentrations. IC50 values generated from dose–response non-linear curve fitting after treatment with GemHCl and 4NSG-SLN for (A) (a) PPCL-192 cells, (b) PPCL-135 cells, (c) PPCL-46 cells, and (d) PPCL-68 cells. B Viability of (I) PPCL-192, (II) PPCL-135, (III) PPCL-46, and PPCL-68 cells after treatment with GemHCl and 4NSG-SLN. Cell cultures treated with no drug set to 100% (controls) were used as a reference to evaluate the percent viability of vehicle (SLN), GemHCl, and 4NSG-SLN treated PPCL-192, PPCL-135, PPCL-46, and PPCL-68 cultures. Results represent at least three independent experiments and data expressed as mean ± standard deviation (SD), n = 3. Percent viability of (GemHCl vs. 4NSG-SLN, **p < 0.01, ***p < 0.001)