Fig. 3

In vitro experiments exploring the effect of abnormal JMJD2A expression in prostate cancer cells. A Cell activity assay using sensitive and resistant strains of the human prostate cancer cell lines PC3 and DU145; *P < 0.05, **P < 0.01, ***P < 0.001, compared with 0 nM of docetaxel. B JMJD2A overexpression or knockdown plasmids were constructed and transfected into the prostate cancer cells, the efficiency of the transfection detected by western blot; C Apoptosis levels detected by flow cytometry; D Detection of cell proliferation by CCK-8. *P < 0.05, **P < 0.01, ***P < 0.001