Fig. 5

Temporal expression kinetics of UV-DDB in Vector and PTEN-FL cells. (A) Diagram illustrating the timeline of samples collection in post-UVC-irradiation. (B) Relative mRNA expression of the DDB2 was determined by RT-qPCR at the indicated post-irradiated (5 J/m²) time points. The expression values were normalized to an internal control GAPDH. A centered first order polynomial least squares fit was applied to three independent biological replicates to compare fits. The shaded areas represented the error band of 95% CI. (C) Immunoblot detected DDB2 at the indicated time points in the whole-cell extracts. GAPDH was used as a loading control. The band intensities were measured as adjusted density (Adj. Density) relative to GAPDH. (D) Relative mRNA expression of the DDB1 was determined by RT-qPCR at the indicated post-irradiated (5 J/m²) time points. The expression values were normalized to an internal control GAPDH. A centered first order polynomial least squares fit was applied to three independent biological replicates to compare fits. The shaded areas represented the error band of 95% CI. (E) Immunoblot detected DDB1 at the indicated time points in the whole-cell extracts. GAPDH was used as a loading control. The band intensities were measured as adjusted density (Adj. Density) relative to GAPDH.