Fig. 4
Effects of UV light-induced DNA damage on cell cycle and NER activity in EC. A-D. Flow cytometric analyses of cell cycle and unscheduled DNA synthesis (UDS) of either un-irradiated Vector (A) and PTEN-FL cells (B) or 5 J/m2 UVC irradiated Vector (C) and PTEN-FL (D) at 4 h post-irradiation. Cells were analyzed after the incorporation of EdU, followed by AlexaFluor 647 conjugation and DNA staining with PI for the cell cycle (upper panels) and the UDS at the G1 phase (lower panels). The values in the representative scatter plots (upper panel) and histograms (lower panels) were expressed as mean and 95% CI. E. The stacked bar plot described the percentages of G1, S, and G2/M phases cells of three independent biological replicates as median and 95% CI. A two-way ANOVA test was performed to compare multiple groups. F. The plot described the percentages of UDS-positive cells in G1 phase of three independent biological replicates as median and 95% CI. A two-way ANOVA test was performed to compare multiple groups. * P < 0.05, ns, not significant