Fig. 3
Effects of UV light-induced DNA damage on cell viability in EC. (A) A survival plot for Vector and PTEN-FL cells. Cell survivals were determined at 4 h post-irradiation with UVC (254 nm) light by MTT assay. The doses of UVC were transformed into a log scale. A variable slope least square fit was applied to normalized responses of three independent biological replicates to compare fits. The shaded areas represented the error band of 95% CI. (B) The table described the LD50 values of Vector and PTEN-FL cells with 95% CI. (C) Flow cytometric analyses of cell deaths. Cell deaths were determined at 4 h post-irradiation with a 5 J/m2 UVC dose by staining the cells with annexin V conjugated with fluorophore APC and labeling the DNA with PI. The values in the representative scatter plots were expressed as mean and standard deviation. (D) The plot described the percentages of early apoptotic cells of three independent biological replicates as median and 95% CI. A two-way ANOVA test was performed to compare multiple groups. (E) The plot described the percentages of dead cells of three independent biological replicates as median and 95% CI. A two-way ANOVA test was performed to compare multiple groups. * P < 0.05, ** P < 0.01, ns, not significant