Fig. 1
From: A simple and available measurement of onco-sEV dsDNA to protein ratio as a potential tumor marker
Scheme of serum sEVs detection
The detection scheme of NPr and dsDPr was presented in Fig. 1. Serum from healthy individuals and patients with different types of tumors were collected. CEA+ sEVs were enriched using CEA magnetic bead antibody. The absorbance ratio of CEA+ sEVs at A260 and A280 (NPr) was compared using UV spectrophotometry, and the concentration ratio of dsDNA and protein (dsDPr) in CEA+ sEVs was detected by fluorescent dye method