Fig. 2

The effect of ID3 on the radiosensitivity of colorectal cancer was through p53–independent pathway. A Western blotting (left panel) and statistical analysis (right panel) of ID3 and wild-type p53 protein expression in HCT116 cells. Wild-type p53 expression did not change with ID3. B Western blotting (left panel) and statistical analysis (right panel) of ID3 and mutant p53 protein expression in HT-29 cells. Mutant p53 expression did not change with ID3. Experiments were repeated at least three times. Data are expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.001. NC: siRNA control, PC: pcDNA3.1 control, KD: knockdown, OE: overexpress