Skip to main content
Fig. 1 | BMC Cancer

Fig. 1

From: A positive feedback loop between ID3 and PPARγ via DNA damage repair regulates the efficacy of radiotherapy for rectal cancer

Fig. 1

Low expression of ID3 enhanced the radiosensitivity of colorectal cancer cells. A Western blotting (left panel) and statistical analysis (right panel) of ID3 protein expression in colorectal cancer cells with ID3 knockdown and overexpress. β-actin was used as a loading control. The expression of ID3 protein decreased significantly in the ID3 KD group and increased significantly in the ID3 OE group. B Clonogenic assay to assess the effect of ID3 on the clonogenic activity of colorectal cancer cells after irradiation. C Surviving fraction in clonogenic assay. D WST assay to assess the effect of ID3 on the proliferation of colorectal cancer cells after irradiation. E Flow cytometry assay to assess the effect of ID3 on the apoptosis of colorectal cancer cells after irradiation. Experiments were repeated at least three times. Data are expressed as mean ± SD (n = 3). *P < 0.05. NC: siRNA control, PC: pcDNA3.1 control, KD: knockdown, OE: overexpress

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us