Fig. 4

Api5 overexpression in breast acini leads to the epithelial cells acquiring partial EMT-like characteristics. Immunoblotting of lysates collected from (A) day 16 acini and (B) dissociated cells from day 16 acini and grown as monolayer cultures showing differential expression of epithelial and mesenchymal markers in Api5 OE compared to the control. C Violin plot showing corrected cell fluorescence of E-cadherin immunostaining of day 16 control and Api5 OE acini. D Representative image of Vimentin (green) immunostaining of control and Api5 OE day 16 acini. Nuclei were counterstained with Hoechst 33258 (blue). E Corrected cell fluorescence of vimentin quantified using ImageJ and represented as a violin plot. F Representative image of immunostained control and Api5 dissociated cells for Vimentin (green). Nuclei were stained with Hoechst 33258 (blue). G Representative image of GM130 immunostained control and Api5 OE dissociated cells (GM130: green, nuclei: blue). H Golgi area was measured using ImageJ. Statistical analysis was performed using the Mann–Whitney test. *P < 0.05, **P < 0.01, ***P < 0.001and ****P < 0.0001. Data pooled from n > 5 independent experiments