Fig. 3
From: Altered expression of anti-apoptotic protein Api5 affects breast tumorigenesis
Overexpression of Api5 disrupts breast acinar polarity. A Representative image showing basal polarity marker, α6-integrin (green) immunostained in day 16 acini. B Bar diagram showing the percentage of acini with mislocalisation / loss of α6-integrin staining. C Representative image showing basal polarity marker Laminin V immunostaining (green) in day 16 control and Api5 OE acini. D Bar diagram showing the percentage of acini with mislocalisation/ loss of Laminin V staining. E Representative image showing GM130 (green) immunostaining in day 16 control and Api5 OE acini. F Quantification showing percentage of acini with mislocalised GM130 staining. G Representative image showing cell–cell junction marker E-cadherin (green) immunostaining in control and Api5 OE day 16 acini. H Bar diagram showing the percentage of acini with loss of E-cadherin staining. Statistical analysis for the percentage of acini was performed using an unpaired t-test. *P < 0.05, **P < 0.01, ***P < 0.001and ****P < 0.0001. Data pooled from N ≥ 3 independent experiments