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Table 3 The forward (F) and reverse (R) primer sequences for WT and MUT 3′UTR constructs of MDM2

From: Functional role and epithelial to mesenchymal transition of the miR-590-3p/MDM2 axis in hepatocellular carcinoma

MDM2 3’UTR

WT

F 5’ CTAGCGGCTAGTGATATATATAAAGTAAAATTTTCTTTGCAGG 3’

R3’ GCCGATCACTATATATATTTCATTTTAAAAGAAACGTCCAGCT 5’

MDM2 3’UTR

MUT

F5’ CTAGCGGCTAGTGATATATATAAAG–––-TTCTTTGCAGG 3’

R3’ GCCGATCACTATATATATTTC–––-AAGAAACGTCCAGCT 5’