Table 2 RT-qPCR primers used in the RT-qPCR analysis
Gene | Primer sequence |
|---|---|
hsa-mir-590-3p | 5'UAAUUUUAUGUAUAAGCUAGU |
hsa-mir-103a-3p | 5'AGCAGCAUUGUACAGGGCUAUGA |
GAPDH | F: 5’-AAGGTCATCCCTGAGCTGAAC- 3’ R: 5’-ACGCCTGCTTCACCACCTTCT-3’ |
MDM2 | F: 5’-CCCAAGACAAAGAAGAGAGTGTGG-3’ R: 5’-CTGGGCAGGGCTTATTCCTTTTCT-3’ |
N-Cadherin | F: 5’-GCGTCTGTAGAGGCTTCTGGT-3’ R: 5’-TCTGCAGGCTCACTGCTCTC-3’ |
Vimentin | F: 5’-GAACGCCAGATGCGTGAAATG-3’ R: 5’-CCAGAGGGAGTGAATCCAGATTA-3’ |
SNAIL | F: ACTATGCCGCGCTCTTTCCT R: GCTGCTGGAAGGTAAACTCTGG |
SLUG | F: CAAGGCGTTTTCCAGACCCTG R: AAGAAAAAGGCTTCTCCCCCGT |
ZEB1 | F: TGCTGGGAGGATGACACAGG R: CTGCTTCATCTGCCTGAGCTT |
ZEB2 | F: TTCCTGGGCTACGACCATACC R: CAAGCAATTCTCCCTGAAATCC |