Fig. 3

MDM2 is a novel direct target gene of miR-590-3p in HCC. A Differential expression of MDM2 between normal and HCC tissues as obtained from TCGA data using Pan-cancer analysis platform. B The mRNA levels of MDM2 in HepG2 cells transfected with miR-590-3p in mimic compared to the negative control. C The sequence alignment between MDM2 and miR-590-3p. D Relative luciferase activity of miR-590-3p mimic or negative control on the WT or MUT versions of MDM2. Data represents the mean ± SEM. (N = 3 for qPCR, N = 2 for luciferase assay). P-value was depicted using Student’s t-test. (* P < 0.05, ** P < 0.01, *** P < 0.001)