Fig. 2

miR-590-3p suppresses cellular clonogenicity, migration and EMT in HepG2 cells. A The transfection efficiency of miR-590-3p mimics compared to negative control B Overexpressing miR-590-3p significantly decreased the number of HepG2 colonies compared to the negative control. C Overexpressing miR-590–3 showed a low but significant reduction in the number of migrated cells as compared to the negative control in the HepG2 cell line. D overexpressing miR-590-3p caused a significant decrease in the mRNA levels of SNAIL, SLUG, ZEB1, and ZEB2. E N-cadherin levels were significantly lower in miR-590-3p mimics transfected cells, while Vimentin levels were not significantly changed. Images were taken at 20X magnification using a fluorescent microscope and counted using Fiji Image J software. Data represents the mean ± SEM. (N = 2 for qPCR, N = 3 for Transwell and colony assay). P-value was depicted using Student’s t test. (* P < 0.05, ** P < 0.01, *** P < 0.001)