Table 1 Relationship between clinicopathological characteristics and lymph node metastasis (LNM) in 158 pT1b ESCCa patients
Clinicopathological characteristics | Total | Invasion depth | LNM | Univariate logistic regression | |||||
|---|---|---|---|---|---|---|---|---|---|
sm1 | sm2 | sm3 | No (%) | Yes (%) | Odds ratio | 95% CI | P | ||
Lymphovascular invasion | |||||||||
HE staining | |||||||||
 HE-VIb +  | 7 | 0 | 3 | 4 | 5 (71.4) | 2 (28.6) | 1.005 | 0.188–5.377 | 0.996 |
 HE-VI- | 151 | 15 | 33 | 103 | 108 (71.5) | 43 (28.5) | 1 |  |  |
 HE–LIc +  | 35 | 4 | 5 | 26 | 17 (48.6) | 18 (51.4) | 3.765 | 1.711–8.282 | 0.001 |
 HE–LI- | 123 | 11 | 31 | 81 | 96 (78) | 27 (22) | 1 |  |  |
Elastin staining + CK (AE1/AE3) IHC d | |||||||||
 E-VIe +  | 109 | 4 | 24 | 81 | 74 (67.9) | 35 (32.1) | 1.845 | 0.827–4.116 | 0.135 |
 E-VI- | 49 | 11 | 12 | 26 | 39 (79.6) | 10 (20.4) | 1 |  |  |
 Peritumoral E-VI +  | 62 | 4 | 15 | 43 | 41 (66.1) | 21 (33.9) | 1.537 | 0.763–3.094 | 0.229 |
 Peritumoral E-VI- | 96 | 11 | 21 | 64 | 72 (75.0) | 24 (25.0) | 1 |  |  |
CD31 + CK (AE1/AE3) D-IHC f | |||||||||
 CD31-VIg +  | 40 | 1 | 8 | 31 | 25 (62.5) | 15 (37.5) | 1.760 | 0.821–3.773 | 0.146 |
 CD31-VI- | 118 | 14 | 28 | 76 | 88 (74.6) | 30 (25.4) | 1 |  |  |
 Peritumoral CD31-VI +  | 32 | 1 | 6 | 25 | 21 (65.6) | 11 (34.4) | 1.417 | 0.619–3.247 | 0.409 |
 Peritumoral CD31-VI- | 126 | 14 | 30 | 82 | 92 (73) | 34 (27) | 1 |  |  |
D2-40 + CK (AE1/AE3) D-IHC | |||||||||
 D2-40-LIh +  | 78 | 6 | 16 | 56 | 51 (65.4) | 27 (34.6) | 1.824 | 0.904–3.680 | 0.094 |
 D2-40-LI- | 80 | 9 | 20 | 51 | 62 (77.5) | 18 (22.5) | 1 |  |  |
 Peritumoral D2-40-LI +  | 67 | 6 | 16 | 47 | 41 (61.2) | 26 (38.8) | 2.403 | 1.187–4.863 | 0.015 |
 Peritumoral D2-40-LI- | 91 | 9 | 22 | 60 | 72 (79.9) | 19 (20.1) | 1 |  |  |
 L0 i | 80 | 9 | 20 | 51 | 62(77.5) | 18 (22.5) | 1 |  |  |
 L1 | 50 | 3 | 8 | 39 | 39 (78.0) | 11 (22.0) | 0.881 | 0.379–2.051 | 0.947 |
 L2 | 28 | 3 | 8 | 17 | 12 (42.9) | 16 (57.1) | 6.889 | 2.540–18.685 |  < 0.001 |
Age | |||||||||
  ≥ 60 | 58 | 7 | 19 | 32 | 42 (72.4) | 16 (27.6) | 0.933 | 0.454–1.916 | 0.849 |
  < 60 | 100 | 8 | 17 | 75 | 71 (71.0) | 29 (29.0) | 1 |  |  |
Sex | |||||||||
 Male | 43 | 5 | 9 | 29 | 33 (76.7) | 10 (23.3) | 0.693 | 0.308–1.559 | 0.375 |
 Female | 115 | 10 | 27 | 78 | 80 (69.6) | 35 (30.4) | 1 |  |  |
Degree of differentiation | |||||||||
 Well | 31 | 2 | 6 | 23 | 27 (87.1) | 4 (12.9) | 1 |  |  |
 Moderate | 60 | 7 | 18 | 35 | 42 (70.0) | 18 (30.0) | 2.893 | 0.883–9.475 | 0.079 |
 Poor | 40 | 3 | 7 | 30 | 25 (62.5) | 15 (37.5) | 4.050 | 1.184–13.853 | 0.026 |
 Basaloid | 19 | 1 | 5 | 13 | 14 (73.7) | 5 (26.3) | 2.411 | 0.557–10.429 | 0.239 |
 Sarcomatoid | 8 | 2 | 0 | 6 | 5 (62.5) | 3 (37.5) | 4.050 | 0.686–23.901 | 0.123 |
Tumor location | |||||||||
 Upper thoracic | 31 | 1 | 10 | 20 | 24 (77.4) | 7 (22.6) | 0.781 | 0.303–2.015 | 0.609 |
 Middle thoracic | 103 | 14 | 20 | 69 | 75 (72.8) | 28 (27.2) | 1 |  |  |
 Lower thoracic | 24 | 0 | 6 | 18 | 14 (58.3) | 10 (41.7) | 1.913 | 0.762–4.802 | 0.167 |
Macroscopic type | |||||||||
 Plaque-like | 85 | 8 | 15 | 62 | 59 (69.4) | 26 (30.6) | 1 |  |  |
 papillary | 18 | 2 | 5 | 11 | 16 (88.9) | 2 (11.1) | 0.284 | 0.061–1.324 | 0.109 |
 Erosive | 31 | 2 | 14 | 15 | 23 (74.2) | 8 (25.8) | 0.789 | 0.3121–1.995 | 0.617 |
 Ulcerative | 9 | 1 | 1 | 7 | 5 (55.6) | 4 (44.4) | 1.185 | 0.451–7.313 | 0.402 |
 Intraluminal mass | 15 | 2 | 1 | 12 | 10 (66.7) | 5 (33.3) | 1.135 | 0.353–3.650 | 0.832 |
Depth of tumor invasion j | |||||||||
 sm1 | 15 | - |  |  | 12 (80.0) | 3 (20.0) | 1 |  |  |
 sm2 | 36 | - |  |  | 28 (77.8) | 8 (22.2) | 1.143 | 0.258–5.067 | 0.861 |
 sm3 | 107 | - |  |  | 73 (68.2) | 34 (31.8) | 1.863 | 0.493–7.037 | 0.359 |
Tumor thickness | |||||||||
  ≥ 3000 μm | 125 | 8 | 20 | 97 | 85 (68.0) | 40 (32.0) | 2.635 | 0.947–7.331 | 0.063 |
  < 3000 μm | 33 | 7 | 16 | 10 | 28 (84.8) | 5 (15.2) | 1 |  |  |
Submucosal invasion thickness | |||||||||
  ≥ 2000 μm | 94 | 3 | 8 | 83 | 62 (66.0) | 32 (34.0) | 2.025 | 0.963–4.259 | 0.063 |
  < 2000 μm | 64 | 12 | 28 | 24 | 51 (79.7) | 13 (20.3) | 1 |  |  |