Fig. 2

Optimization of the ddPCR system. A Optimization of annealing temperature. The plasmid based standard DNA template (left panel) or enzyme-free water (right panel) were used for amplification. A set of gradient temperatures were labeled on the top of figures. Eight reactions are separated by yellow lines, the amplitude of fluorescent readouts, and positive (blue) and negative (gray) droplets are separated by the threshold (pink line). B Optimization of the concentration of primers and probe. The standard DNA template (left panel) or enzyme-free water (right panel) were used for amplification. A series of different combinations of primers and probe were labeled on the top of figures