Fig. 1

Presence of anti-ING1 antibodies in sera of patients with CRC. The reactivity of anti-ING1 antibodies against ING1 protein were examined by western blot analysis. Glutathione-S-transferase (GST) (lane 1) and GST-ING1 proteins (lane 2) were electrophoresed on sodium dodecyl sulfate–polyacrylamide gels. Anti-GST antibody (Rockland, Gilbertsville, PA), serum from healthy donors (HDs), or sera from patients with colorectal cancer (CRC) (CRC #1 and #2) were used as primary antibodies. One of the 2 independent repeat experiments is shown. Molecular sizes are shown at the left. Asterisks represent the degradation products of GST-ING1 protein. The full blot images are shown in Additional file 2: Figure S1