Fig. 6

Effects of antagonize GPR84 under 200 μM FFA C8:0 stimulation on biological behavior of PC3 cells. A The mRNA expression level of GPR84 in PC3 cells after 200 μM FFA C8:0 stimulation was detected by qRT-PCR. B CCK8 assay was used to detect the proliferation ability of PC3 cells after antagonizing GPR84 under 200 μM FFA C8:0 stimulation. C The effect of antagonizing GPR84 under 200 μM FFA C8:0 stimulation on the colony formation ability of PC3 was detected by plate Colony assay. D The effect of antagonizing GPR84 under 200 μM FFA C8:0 stimulation on the healing ability of PC3 cells was detected by scratch experiment (40 ×). E–F The effect of antagonizing GPR84 under 200 μM FFA C8:0 stimulation on the invasion (E) and migration (F) ability of PC3 cells was detected by Transwell assay (200 ×). G The mRNA expression level of KLF7 in PC3 cells after antagonizing GPR84 under 200 μM FFA C8:0 stimulation was detected by qRT-PCR. H The protein expression levels of KLF7, IL-6, and p21 in PC3 cells after antagonizing GPR84 under 200 μM FFA C8:0 stimulation were detected by Western Blot. I The secretion level of IL-6 in PC3 cells after antagonizing GPR84 under 200 μM FFA C8:0 stimulation were detected by ELISA. one-way ANOVA test, *P < 0.05, ***P < 0.001 the difference was statistically significant