Fig. 5

Effects of down-regulated KLF7 under 200 μM FFA C8:0 stimulation on biological behavior of PC3 cells. A CCK8 assay was used to detect the proliferation ability of PC3 cells after down-regulation of KLF7. B The effect of down-regulated KLF7 on the colony formation ability of PC3 was detected by plate Colony assay. C-D The effect of down-regulated KLF7 on the invasion (C) and migration (D) ability of PC3 cells was detected by Transwell assay (200 ×). E The mRNA expression levels of KLF7, IL-6, p21 and MMP2 in PC3 cells after down-regulating KLF7 was detected by qRT-PCR. F The secretion level of IL-6 in PC3 cells after down-regulating KLF7 were detected by ELISA. G The protein expression levels of KLF7, IL-6, p21 and MMP2 in PC3 cells after down-regulating KLF7 were detected by Western Blot. H CCK8 assay was used to detect the proliferation ability of PC3 cells after down-regulated KLF7 under 200 μM FFA C8:0 stimulation. I The effect of down-regulated KLF7 under 200 μM FFA C8:0 stimulation on the colony formation ability of PC3 was detected by plate Colony assay. J-K The effect of down-regulated KLF7 under 200 μM FFA C8:0 stimulation on the invasion (J) and migration (K) ability of PC3 cells was detected by Transwell assay (200 ×). L The mRNA expression levels of IL-6, p21 and MMP2 in PC3 cells after down-regulated KLF7 under 200 μM FFA C8:0 stimulation were detected by qRT-PCR. M The protein expression levels of IL-6, p21 and MMP2 in PC3 cells after down-regulated KLF7 under 200 μM FFA C8:0 stimulation were detected by Western Blot. N The secretion level of IL-6 in PC3 cells after down-regulated KLF7 under 200 μM FFA C8:0 stimulation were detected by ELISA. t test, *P < 0.05 the difference was statistically significant