Fig. 4

Effects of up-regulated KLF7 on biological behavior of PC3 cells. A CCK8 assay was used to detect the proliferation ability of PC3 cells after up-regulation of KLF7. B The effect of up-regulated KLF7 on the colony formation ability of PC3 was detected by plate Colony assay. C The effect of up-regulated KLF7 on the healing ability of PC3 cells was detected by scratch experiment (40 ×). D-E The effect of up-regulated KLF7 on the invasion (D) and migration (E) ability of PC3 cells was detected by Transwell assay (200 ×). F The mRNA expression levels of KLF7, IL-6, p21 and MMP2 in PC3 cells after up-regulating KLF7 were detected by qRT-PCR. G The protein expression levels of KLF7, IL-6, p21 and MMP2 in PC3 cells after up-regulating KLF7 were detected by Western Blot. H The secretion level of IL-6 in PC3 cells after up-regulating KLF7 were detected by ELISA. t test, *P < 0.05, **P < 0.01 the difference was statistically significant